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anti sirt2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti sirt2
    Anti Sirt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirt2/SirT2+Rabbit+mAb/bio_rxiv__64898__2026__03__13__711520-87-2-9
    Average 95 stars, based on 83 article reviews
    anti sirt2 - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Unraveling Network Pharmacology-Based Therapeutics of Anthranilate Sulfonamides via Sirtuins/FOXO3a Cascade in Alzheimer's Disease.
    Article Snippet: .. Then, the membranes were incubated with primary antibodies (i.e., SIRT1 (RRID: AB_10999470), SIRT2 (RRID: AB 2636961), SIRT3 (RRID: AB_2188622), FOXO3a (RRID: AB_2636990), BAX (RRID: AB_10557411), BCL- 2 (RRID: AB_2744528), SOD2 (RRID: AB_2636921), CAT (RRID: AB_2798079), and β- actin (RRID: AB_330288) from Cell Signaling Technology, MA, USA) at 4°C overnight. .. The probe membranes were washed with TBST, followed by incubation with horseradish peroxidase (HRP) conjugated secondary antibodies at room temperature for 1 h. Bound antibody was detected by Immobilon ECL Ultra Western HRP Substrate (Merck Millipore, Darmstadt, Germany).

    Article Title: Unraveling Network Pharmacology‐Based Therapeutics of Anthranilate Sulfonamides via Sirtuins/ FOXO3a Cascade in Alzheimer's Disease
    Article Snippet: .. Then, the membranes were incubated with primary antibodies (i.e., SIRT1 (RRID: AB_10999470), SIRT2 (RRID: AB 2636961), SIRT3 (RRID: AB_2188622), FOXO3a (RRID: AB_2636990), BAX (RRID: AB_10557411), BCL‐2 (RRID: AB_2744528), SOD2 (RRID: AB_2636921), CAT (RRID: AB_2798079), and β‐actin (RRID: AB_330288) from Cell Signaling Technology, MA, USA) at 4°C overnight. .. The probe membranes were washed with TBST, followed by incubation with horseradish peroxidase (HRP) conjugated secondary antibodies at room temperature for 1 h. Bound antibody was detected by Immobilon ECL Ultra Western HRP Substrate (Merck Millipore, Darmstadt, Germany).

    Article Title: MATERNAL OBESITY MODULATES FOXO1 ACTIVATION AND ADIPOGENESIS IN NEONATAL MESENCHYMAL STEM CELLS
    Article Snippet: 60 μg of proteins was run in 10% Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for 1.5 hours at 120 mV, transferred to a nitrocellulose membrane (Bio-Rad) and blocked with 5% powdered non-fat milk (Friesland Campina) or BSA (Sigma-Aldrich) in 1x Tris-buffered saline plus 0.1% Tween T (TBST), for one hour. .. After blocking, the membranes were incubated overnight at 4°C with antibodies for FOXO1 (Invitrogen, 1:500), acetyl-FOXO1 Lys 294 (Invitrogen, 1:1000), SIRT2 (Cell Signaling, 1:500), PPARγ (Cell Signaling, 1:250) and β-actin (Cell Signaling, 1:10000) and α-tubulin (Proteintech, 1:1000). .. Thereafter, membranes were washed and incubated with secondary antibodies conjugated with HRP for one hour at room temperature (Rabbit Anti-Mouse and Goat Anti-Rabbit, Dako, Agilent, 1:5000).

    Western Blot:

    Article Title: Deacetylation of HSC70 by SIRT2 promotes chaperone mediated autophagy
    Article Snippet: pCMV3-HSC70-HA (#HG11329-CY) and pCMV3-SIRT2-HA (HG10830-CH) plasmid were obtained from SinoBiological. pSIN-PAmCherry-KFERQ-NE was a gift from Shu Leong Ho (Addgene plasmid # 102,365; http://n2t.net/addgene:102365 ; RRID:Addgene_102365). .. Primary Antibodies from Invitrogen: HSC70 (MA1-26078); Cell Signaling Technologies: SIRT2 (#12650), Santa Cruz Biotechnology: HA-HRP (sc-7392); Flag-horse radish peroxidase (Sigma #A8592) was used to immunoblot for Flag-conjugated proteins at 1:5000 dilution. .. Precision Plus ProteinTM All Blue Prestained Protein Standards (Biorad #1610393) was used for molecular size markers for immunoblotting (Western blot).

    Blocking Assay:

    Article Title: MATERNAL OBESITY MODULATES FOXO1 ACTIVATION AND ADIPOGENESIS IN NEONATAL MESENCHYMAL STEM CELLS
    Article Snippet: 60 μg of proteins was run in 10% Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for 1.5 hours at 120 mV, transferred to a nitrocellulose membrane (Bio-Rad) and blocked with 5% powdered non-fat milk (Friesland Campina) or BSA (Sigma-Aldrich) in 1x Tris-buffered saline plus 0.1% Tween T (TBST), for one hour. .. After blocking, the membranes were incubated overnight at 4°C with antibodies for FOXO1 (Invitrogen, 1:500), acetyl-FOXO1 Lys 294 (Invitrogen, 1:1000), SIRT2 (Cell Signaling, 1:500), PPARγ (Cell Signaling, 1:250) and β-actin (Cell Signaling, 1:10000) and α-tubulin (Proteintech, 1:1000). .. Thereafter, membranes were washed and incubated with secondary antibodies conjugated with HRP for one hour at room temperature (Rabbit Anti-Mouse and Goat Anti-Rabbit, Dako, Agilent, 1:5000).



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